EpiGLO™-Tox HT

The In Vitro Standardized and Validated
ATP Bioluminescence Intestinal Toxicity
Testing and High-Throughput Screening Platform



EpiGLO™-Tox HT

A Standardized and Validated In Vitro Bioluminescence Intestinal Cell Toxicity Assay

Cell Population Species Medium Formulation Catalog Number Quantity
Intestinal cells (adherent) Any User defined KEG-T96A-4 1 Kit
Intestinal cells (non-adherent) Any User defined KEG-T96NA-4 1 Kit


Epiglottis™-Tox HT: A standardized and validated ATP bioluminescence intestinal toxicity testing and screening platform



  • Detecting and measuring potential toxicity to primary intestinal cells and intestinal cell lines .
  • Specifically designed to screen and test for in vitro intestinal toxicity.
  • High throughput capability during in vitro ADME/Tox screening.
  • Allows drug and species ranking of toxicity.
  • Incorporates proven, standardized and validated ATP bioluminescence technology, the most sensitive signal detection system available.
  • Includes all reagents to calibrate and standardize the assay.
  • Measurement assurance parameters included to ensure the assay is working correctly.
  • Reliably compare results over time and between drug lots.
  • Assay kits include everything needed to perform the readout.
  • 96-Well plates included with all kits and available for adherent or non-adherent cells.
  • Available as 2- or 4-plate assay kits. Custom sizes also available.
  • Designed for multiplexing capability.
  • Rapid turnaround.
  • Simple and time efficient.

Principle of ATPBioluminescence Cell Proliferation/Cytotoxicity Assays

All ATP bioluminescence assays kits from Preferred Cell Systems™ include an ATP standard(s) and controls.  Performing the ATP standard curve and controls is highly recommended. The controls are used to calibrate the luminescence plate reader and the ATP standard is used to standardize the assay. The values obtained from the standard curve and controls (see diagram below) are then compared to the measurement assurance parameters that are provided in the instruction manual. If the values obtained are within the ranges provided by the measurement assurance parameters, you, the user, have successfully performed the proficiency testing to ensure that you have not only performed the assay correctly, but that the results you obtain can be trusted.

No additional proficiency testing is required if the calibration and standardization procedure is performed. The values you obtain from each calibration and standardization can be logged and used for certification that the assay has been performed correctly and that the results are trustworthy.

Calibration and standardization of an ATP bioluminescence assay from Preferred Cell Systems™ - Measurement Assurance

EpiGLO™-Tox HT can be used with isolated primary intestinal cell types from different species. The assay can also be used with intestinal cell lines. 

EpiGLO™-Tox HT assays are available with 96-well plates. Upon required, 384-well plates are also available.

For Research Use Only. Not for clinical diagnostic use.
Luminescence or multimode plate reader with "glow" luminescence measuring capability.

  • IMDM base medium for dilution of ATP standard.
  • ATP standard.
  • ATP controls.
  • ATP Enumeration Reagent.
  • Sterile, solid white 96-well plates. Available for adherent or non-adherent cells.
  • Non-sterile, solid, white 96-well plates.
  • Sterile, adhesive foil covers to maintain sterility of unused wells.
EpiGLO™-Tox HT is flexible so that the investigator can use there own growth medium to culture the cells.


Please visit the Instructional Videos page

Download the EpiGLO™-Tox HT Technical Manual
Download the ATP Optimization Kit Protocol for First-Time Users
Download Luminometer Setup and RLU to ATP Conversion
Download Certificate of Analysis (CoAs) for ATP Enumeration Reagent (ATP-ER)
Download Certificate of Analysis for ATP Stanadrds
Download Certificate of Analysis for ATP Controls
Download Certificate of Analysis of ATP Reconstitution Reagent
Download Certificate of Analysis for Sterile 96-Well Plates
Download Certificate of Analysis for Non-Sterile, 96-Well Plates
Download Certificate of Analysis for Sealing Films
Download Certificate of Analysis for IMDM