ImmunoGlo™-Tox HT

A Standardized and Validated In Vitro Bioluminescence Immunotoxicity Platform for T‑Lymphocytes

Cell Population	Species	Medium Formulation	Catalog Number	Quantity
Immune cells	Multiple / Any	ImmunoGro™ Base Medium	KM1-T96-4	1 Kit

ImmunoGlo™-Tox HT TCP

A Standardized and Validated In Vitro Bioluminescence Immunotoxicity Platform for T‑Lymphocytes

Cell Population	Species/Tissue	ImmunoGro™ Medium Formulation	Catalog Number	Quantity
Low Serum
T-lymphocytes	Human / Any	Low Serum + IL-2	KM1-T96CS1-4H	1 Kit
T-lymphocytes	Human / Any	Low Serum + CD3 + CD28	KM1-T96CS2-4H	1 Kit
T-lymphocytes	Human / Any	Low Serum + IL-2 + CD3 + CD28	KM1-T96CS3-4H	1 Kit
Serum Free
T-lymphocytes	Human / Any	Serum-free + IL-2	KM1SF-T96CS1-4H	1 Kit
T-lymphocytes	Human / Any	Serum-free + CD3 + CD28	KM1SF-T96CS2-4H	1 Kit
T-lymphocytes	Human / Any	Serum-free + IL-2 + CD3 + CD28	KM1SF-T96CS3-4H	1 Kit

ImmunoGlo™ is trademark by Preferred Cell Systems

ImmunoGlo™-Tox HT TCP

A Standardized and Validated In Vitro Bioluminescence Immunotoxicity Platform for T‑Lymphocytes

Cell Population	Species/Tissue	ImmunoGro™ Medium Formulation	Catalog Number	Quantity
T-lymphocytes	Primate / Any	Low serum	KM1-T96CS1-4Pr	1 Kit

ImmunoGlo™ is trademark by Preferred Cell Systems

ImmunoGlo™-Tox HT TCP

A Standardized and Validated In Vitro Bioluminescence Immunotoxicity Platform for T‑Lymphocytes

Cell Population	Species/Tissue	ImmunoGro™ Medium Formulation	Catalog Number	Quantity
T-lymphocytes	Rat / Any	Low serum	KM1-T96CS1-4R	1 Kit

ImmunoGlo™ is trademark by Preferred Cell Systems

ImmunoGlo™-Tox HT TCP

A Standardized and Validated In Vitro Bioluminescence Immunotoxicity Platform for T‑Lymphocytes

Cell Population	Species/Tissue	ImmunoGro™ Medium Formulation	Catalog Number	Quantity
T-lymphocytes	Mouse / Any	Low serum	KM1-T96CS1-4M	1 Kit

ImmunoGlo™ is trademark by Preferred Cell Systems

ImmunoGlo™-Tox HT BCP

A Standardized and Validated In Vitro Bioluminescence Immunotoxicity Platform for T‑Lymphocytes

Cell Population	Species	Medium Formulation	Catalog Number	Quantity
B-lymphocytes	Human / Any	Low Serum +Ligands+IL4	KM1-B96C2-4H	1 Kit
B-lymphocytes	Human / Any	Low Serum+Conjugates+Ligands+IL-4	KM1-B96C3-4H	1 Kit

• ImmunoGlo™-Tox HT is a general use functional immune cell proliferation assay where the investigator employs their own culture reagents and protocol for any type of immune cell.
• ImmunoGlo™ Tox HT TCP Assay Kits include specific cocktails to stimulate functional T-lymphocytes.
• ImmunoGlo™ Tox HT BCP is specific for stimulating functional human B-lymphocytes.

• In vitro high-throughput immunotoxicity screening.
• In vitro high throughput lymphocyte proliferation screening.
• Specialized toxicity assays for T- and B-lymphocytes.
• Cellular immune response screening.
• Development of drugs and immunotherapies using gene targeting, e.g. CRISPR-Cas9.

• Non-radioactive assay incorporating proven high-throughput ATP bioluminescence technology.
• Instrument-based, quantitative and fully standardized allowing comparison of results over time.
• Can be validated for GLP studies.
• More sensitive than WST-1, CSFE and other absorbance or fluorescence assays.
• Available in 96- or 384-well plate formats.
• Multiplexing capability to incorporate flow cytometry, cytokine release and other assay readouts for additional information from the same sample.
• Simple, time efficient and cost effective. 

All ATP bioluminescence assays kits from Preferred Cell Systems™ include an ATP standard(s) and controls.  Performing the ATP standard curve and controls is highly recommended. The controls are used to calibrate the luminescence plate reader and the ATP standard is used to standardize the assay. The values obtained from the standard curve and controls (see diagram below) are then compared to the measurement assurance parameters that are provided in the instruction manual. If the values obtained are within the ranges provided by the measurement assurance parameters, you, the user, have successfully performed the proficiency testing to ensure that you have not only performed the assay correctly, but that the results you obtain can be trusted.

No additional proficiency testing is required if the calibration and standardization procedure is performed. The values you obtain from each calibration and standardization can be logged and used for certification that the assay has been performed correctly and that the results are trustworthy.


 

ImmunoGlo™-Tox HT can be used for multiple species, including:

• Human
• Primate
• Rat
• Mouse

• Peripheral blood mononuclear cells (PBMC)
• Primary lymphocyte sub-populations
• Primary organs and tissues containing immune cells
• Lymphocyte cell lines

For Research Use Only.

Luminescence or multimode plate reader with "glow" luminescence measuring capability.

ImmunoGlo™-Tox HT

• Base medium for ATP standard dilution
• ATP standard
• ATP high and low controls
• ATP Enumeration Reagent
• Sterile, 96- or 384-well culture plates
• Non-sterile, 96- or 384-well plates

ImmunoGlo™-Tox HT TCP

• ImmunoGlo™ TCPMaster Mix for requested T-lymphocyte stimulation
• Base medium for ATP standard dilution
• ATP standard
• ATP high and low controls
• ATP Enumeration Reagent
• Sterile, 96- or 384-well culture plates
• Non-sterile, 96- or 384-well plates

ImmunoGlo™-Tox HT BCP

• ImmunoGlo™ BCP Master Mix for requested B-lymphocyte stimulation
• Base medium for ATP standard dilution
• ATP standard
• ATP high and low controls
• ATP Enumeration Reagent
• Sterile, 96- or 384-well culture plates
• Non-sterile, 96- or 384-well plates

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Download the ImmunoGlo™-Tox HT Technical Manual
Download the ATP Optimization Kit Protocol for First-Time Users
Download Luminometer Setup and RLU to ATP Conversion
Download Certificate of Analysis (CoAs) for ATP Enumeration Reagent (ATP-ER)
Download Certificate of Analysis for ATP Stanadrds
Download Certificate of Analysis for ATP Controls
Download Certificate of Analysis of ATP Reconstitution Reagent
Download Certificate of Analysis for Sterile 96-Well Plates
Download Certificate of Analysis for Non-Sterile, 96-Well Plates
Download Certificate of Analysis for Sealing Films
Download Certificate of Analysis for IMDM