MultiCellGro™
A Unique Cell-Based, Multi-Stem Cell, Multi-Lineage, Fluorescence Assay for Hematopoietic Cells
Buy MultiCellGro™
MultiCellGro™
A Multi-Stem Cell, Multi-Lineage, Fluorescence Assay for Hematopoietic Cells
| Cell Population | Species | Formulation | Catalog Number | Quantity | |
|---|---|---|---|---|---|
| Multi-Stem Cell Low Serum | |||||
| Multi-Lineage | Human | Low serum | MCG-K5-1H | 1 Kit | |
| Multi-Lineage | Mouse | Low serum | MCG-K5-1M | 1 Kit | |
| Multi-Lineage | Primate | Low serum | MCG-K5-1Pr | 1 Kit | |
| Multi-Lineage | Rat | Low serum | MCG-K5-1R | 1 Kit | |
| Multi-Lineage | Dog | Low serum | MCG-K5-1C | 1 Kit | |
| Multi-Stem Cell Serum Free | |||||
| Multi-Lineage | Human | Serum-free | MCGSF-K5-1H | 1 Kit | |
| Multi-Lineage | Mouse | Serum-free | MCGSF-K5-1M | 1 Kit | |
| Multi-Lineage | Primate | Serum-free | MCGSF-K5-1Pr | 1 Kit | |
Uses
- For any type of lympho-hematopoietic research and cellular therapy application where multi-lineage cell proliferation and the ability to phenotypically characterize cells by flow cytometry is required.
- Using a novel growth factor/cytokine cocktail, MultiCellGro™ induces and stimulates different stem and progenitor cells from multiple lineages into proliferation.
- Quantitatively measures cell proliferation using a fluorescence or multimode plate reader (380-400nm excitation/505nm emission).
- The non-lytic/non-destructive fluorescence reagent allows the addition of fluorochrome-conjugated antibodies and phenotypic analysis of stem and multi-lineage-specific cells to be detected by flow cytometry.
- Can also be used with bioluminescence assays.
- Results in 4-7 days depending on species. Incubation times can be extended.
- Simple, time efficient and cost effective.
- Human
- Mouse
MultiCellGro™ is also available serum-free for:
- Human
- Mouse
- Stem cells, CD133 and/or CD34
- Eythropoietic cells: P-BFU (BFU-E equivalent), CD235a (Glycophorin-A )
- Myelomonocytic cells: P-GM (GM-CFC equivalent), CD14 and/or CD15
- Megakaryopoietic cells: P-Mk (Mk-CFC equivalent), CD41 and/or CD61
- T-lymphocytes: P-Tcells (T-CFC equivalent), CD3 , CD4 and/or CD8
- B-lymphocytes: P-Bcells (B-CFC equivalent), CD19
| Characteristics | HALO: | HemoGLO™ | HemoFLUOR™ | MultiCellGro™ | HemoLIGHT™ |
|---|---|---|---|---|---|
| Uses: | For all applications where a standardized and validated assay is required. Basic and stem cell research. Veterinary research. Cellular therapy. Hematotoxicity testing and screening |
For all hematopoietic viability and cell proliferation applications. Basic and stem cell research. Veterinary research. Limited cellular therapy. |
For all hematopoietic viability and cell proliferation applications. Basic and stem cell research. Veterinary research. Limited cellular therapy. |
For primitive lympho-hematopoietic stem cell applications. Basic and stem cell research. Veterinary research. Limited cellular therapy. |
For all hematopoietic viability and cell proliferation applications. Basic and stem cell research. Veterinary research. Limited cellular therapy. |
| Assay availability: | 1 kit | 1 kit | 1 kit | 1 kit | 1 kit |
| Number of samples: | Depends on number of replicates required(1) |
Depends on number of replicates required(1) |
Depends on number of replicates required(1) |
Depends on number of replicates required(1) |
Depends on number of replicates required(1) |
| Type of assay(2): | Suspension Expansion Culture™ (SEC™) |
Suspension Expansion Culture™ (SEC™) |
Suspension Expansion Culture™ (SEC™) |
Suspension Expansion Culture™ (SEC™) |
Suspension Expansion Culture™ (SEC™) |
| Assay format: | 96-well plate. 384-well plate upon request(3) | 96-well plate. | 96-well plate. | 96-well plate | 96-well plate |
| Throughput: | High throughput | High throughput capability | High throughput capability | High throughput capability | High throughput capability |
| Cell functionality detected: | Cell viability/ cell proliferation/ cytotoxicity |
Cell viability/ cell proliferation |
Cell viability/ cell proliferation |
Cell viability/ cell proliferation |
Cell viability/ cell proliferation |
| Biochemical principle: | Intracellular ATP, luciferin/luciferase |
Intracellular ATP, luciferin/luciferase |
Resazurin to resorufin (fluorescent) |
Resazurin to resorufin (fluorescent) |
Tetrazolium WST-1 reduction to yellow formazan |
| Wavelength (filters): | None. Glow luminescence | None. Glow luminescence | Excitation:560nm. Emission: 590nm | Excitation:560nm. Emission: 590nm | Absorbance at 440nm |
| Signal development time: | 10 minutes | 10 minutes | 1-3 hours | 1-3 hours | 1-4 hours |
| Readout: | Luminescence plate reader(4) |
Luminescence plate reader(4) |
Fluorescence plate reader (4) |
Fluroescence plate reader(4) |
Absorbance plate reader(4) |
| Readout endpoint: | Relative luminescence units (RLU) with conversion to ATP concentrations |
Relative luminescence units (RLU) with optional(5) conversion to ATP concentrations |
Relative fluorescence units (RFU) |
Relative fluorescence units (RFU) |
Relative absorbance units (RAU) |
| Subjectivity: | Non-subjective | Non-subjective | Non-subjective | Non-subjective | Non-subjective |
| Cell types detected: | Stem and progenitor cells |
Stem and progenitor cells |
Stem and progenitor cells |
Primitive lympho- hematopoietic stem cells |
Stem and progenitor cells |
| Species: | Human. Primate. Horse. Pig. Sheep. Dog. rat. Mouse | Human. Primate. Horse. Pig. Sheep. Dog. rat. Mouse | Human. Primate. Horse. Pig. Sheep. Dog. rat. Mouse | Human. Primate. Horse. Pig. Sheep. Dog. rat. Mouse | Human. Primate. Horse. Pig. Sheep. Dog. rat. Mouse |
| MethoCult™ equivalent assays available: | No | Yes | Yes | No | Yes |
| Total culture volume required (6): |
Depends on number of replicates required |
Depends on number of replicates required |
Depends on number of replicates required |
Depends on number of replicates required |
Depends on number of replicates required |
| Suggested number of replicates/culture: |
6-8 or as many as required |
4-8 or as many as required | 4-8 or as many as required | 4-8 or as many as required | 4-8 or as many as required |
| Volume/culture: | 0.1mL | 0.1mL | 0.1mL | 0.1mL | 0.1mL |
| Cell culture time(7): |
3-7 days | 4-7 days | 4-7 days | 4-7 days | 4-7 days |
| Instrument calibration: | Yes(8) | Optional(8) | No | No | No |
| Standardized assay: | Yes(9) | Optional(9) | No | No | No |
| Proficiency testing incorporated into assay: | Yes(10) | Optional(10) | No | No | No |
| Measurement assurance: | Yes(11) | Optional(11) | No | No | No |
| Validation: | Yes(12) | Optional(12) | No | No | No |
(1) = The number of samples tested depends on the number of replicates/sample performed. For example, if 4 replicates/sample are performed, then 24 samples can be analyzed on a single 96-well plate. For 6 replicates, 16 samples. For 8 replicates, 12 replicates. These samples, however, do not have to performed on the same day.
(2) = Suspension Expansion Culture™ (SEC™) Technology takes the place of methylcellulose, allowing easier and faster dispensing.
(3) = Some HALO® assays, including STEMpredict™ and all HALO®-Tox HT assays for hematotoxicity testing are available with 384-well plates upon request.
(4) = Multimode plate readers are also av available that have the capability of luminescence, fluorescence and absorbance.
(5) = HemoGLO™ does not include the reagents to calibrate and standardize the assay. However, these reagents are available separately for this assay.
(6) = The total culture volume will depend on the number of replicates required. For example, if 6 replicates are required, a total volume of 0.8mL should be prepared.
(7) = Culture time depends on the state of fractionation or purification of the source cells, cell population tested and species. In some cases it also depends on the assay itself. For example, STEMpredict™ is a 3-day assay.
(8) = Instrument calibration is performed using the ATP controls included with the assay kit or obtained separately for HemoGLO™.
(9) = Standardization implies performing a standard curve. ATP standards are included in all HALO® assay kits and can be obtained separately for HemoGLO™..
10 = The inclusion of standards and controls allows the results to be compared to measurement assurance parameters (provided) to ensure the assay is working correctly. This allows proficiency testing to be determined directly prior to measuring samples.
11 = Measurement assurance parameters are included in all HALO® assays and when standards and controls are purchased separately for HemoGLO™.
12 = A validated assay allows the user to trust the results obtained. This can only be achieved if standards and controls are included. HALO® and HemoGLO™ have been validated. The validation parameters are included for all HALO® assays and included when the optional standardization kit is purchased for HemoGLO™. Standards and controls allow for in-house validation.
- MultiCellGro™ Master Mix available serum-free or low serum in HemoGro™ medium
- GF-AFC Reagent
- Sterile, solid black, 96-well culture plate
- Sterile adhesive foil covers to keep unused wells sterile